{"product_id":"cst-52021t","title":"CST,  52021T, ECT2 (E7V4L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ECT2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ECT2 (E7V4L) Rabbit Monoclonal Antibody (CST #52021) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nECT2 (E7V4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total ECT2 protein.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro325 of human ECT2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEpithelial cell transforming sequence 2 (ECT2), a guanine nucleotide exchange factor (GEF), catalyzes the exchange of GDP for GTP. The nuclear GEF activity of ECT2 is essential for lung tumorigenesis. This transformation requires ECT2-mediated rRNA synthesis. In addition, PKCι-dependent phosphorylation of ECT2 is necessary for the oncogenic activity of this protein (1,2). Furthermore, has been implicated as a cancer driver gene in certain subtypes of liver cancers (3). Research studies also show that, during matrix-directed cell migration, low membrane tension stimulates the formation of caveolae at the rear end of moving cells. ECT2 is recruited to the caveolae and activates RhoA, causing F-actin organization and rear retraction (4).Â\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARHGEF31; ECT2; epithelial cell transforming 2; epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; Protein ECT2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 104\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799788966057,"sku":"52021T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-52021t","provider":"Iright","version":"1.0","type":"link"}