{"product_id":"cst-5213s","title":"CST,  5213S, PCM-1 (G2000) Antibody","description":"Polyclonal Antibody for studying PCM-1. Validated for WB,IP,IF,F. Highly specific and rigorously validated in-house, PCM-1 (G2000) Antibody (CST #5213) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPCM-1 (G2000) Antibody detects endogenous levels of PCM-1 protein. Species cross-reactivity for IF-IC is human only.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region near the carboxyl terminus of human PCM-1 protein. Antibodies were purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Pericentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle-dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centriolar satellites (3). PCM-1 is required for the assembly of several centrosomal proteins, including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhPCM-1; PCM-1; PCM1; pericentriolar material 1; Pericentriolar material 1 protein; pericentriolar material 1, PCM1; PTC4; RET\/PCM-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 228\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799789523113,"sku":"5213S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5213s","provider":"Iright","version":"1.0","type":"link"}