{"product_id":"cst-5249s","title":"CST,  5249S, cPLA2 (D49A7) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying cPLA2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, cPLA2 (D49A7) Rabbit Monoclonal Antibody (CST #5249) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\ncPLA2 (D49A7) Rabbit Monoclonal Antibody detects endogenous levels of total cPLA2 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu245 of human cPLA2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42\/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncalcium-dependent phospholipid-binding protein; CPLA2; cPLA2-alpha; Cytosolic phospholipase A2; GURDP; Lysophospholipase; MGC126350; PA24A; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4; PLA2G4A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 98\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799791423657,"sku":"5249S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5249s","provider":"Iright","version":"1.0","type":"link"}