{"product_id":"cst-5261sc","title":"CST,  5261SC, Mouse Interleukin-10 (mIL-10)","description":"Growth Factors and Cytokines for studying IL10 mouse in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nReconstitution: With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final mIL-10 concentration of greater than 10 μg\/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of mIL-10 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mIL-10 should be greater than 10 μg\/ml.\n\u003cb\u003eFormulation\u003c\/b\u003e\nWith carrier: A 0.22 μm filtered solution of 0.33 mg\/ml mIL-10 in PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-10. Carrier free: A 0.22 μm filtered solution of 0.33 mg\/ml mIL-10 in PBS, pH 7.2.\n\u003cb\u003eStorage\u003c\/b\u003e\nStable at -20°C for 1 year after receipt. Maintain sterility. Do not store frozen.\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nRecombinant mouse IL-10 (mIL-10) Ser19 - Ser178 (Accession # NP_034678) was produced in at Cell Signaling Technology.\n\u003cb\u003eBackground\u003c\/b\u003e\nIL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent infections (6).","brand":"CST","offers":[{"title":"Default Title","offer_id":46800714662057,"sku":"5261SC","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5261sc","provider":"Iright","version":"1.0","type":"link"}