{"product_id":"cst-52632s","title":"CST,  52632S, Aprataxin (D5P3C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying APTX. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Aprataxin (D5P3C) Rabbit Monoclonal Antibody (CST #52632) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAprataxin (D5P3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total aprataxin protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe282 of human aprataxin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAprataxin is a DNA repair protein that resolves DNA lesions (DNA adenylates) caused by abortive ligations in single-strand break repair, double-strand break repair and base excision repair (1). Aprataxin is recruited to sites of DNA damage by PARP1 (2). In the mitochondria, aprataxin and tyrosyl-DNA-phosphodiesterase 1 (TDP1) are required for repair of single strand breaks caused primarily by reactive oxygen species (ROS) (3). The gene for aprataxin, , is defective in the neurodegenerative disorder oculomotor apraxia type 1 (AOA1)(4). Researchers have shown that levels of aprataxin can predict patient response to irinotecan-based treatments in colorectal cancer (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAOA; AOA1; Aprataxin; APTX; AXA1; EAOH; EOAHA; FHA-HIT; FLJ20157; Forkhead-associated domain histidine triad-like protein; MGC1072\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799792963753,"sku":"52632S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-52632s","provider":"Iright","version":"1.0","type":"link"}