{"product_id":"cst-5290s","title":"CST,  5290S, G-alpha(i) Antibody","description":"Polyclonal Antibody for studying GNAI1. Validated for Western Blotting. Highly specific and rigorously validated in-house, G-alpha(i) Antibody (CST #5290) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nG-alpha(i) Antibody detects endogenous levels of total Gα(i) protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg100 of human Gα(i). Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nHeterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines, and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i\/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)\/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP formation (3). Gα(i) causes inhibition of adenylate cyclase, leading to a decrease in cellular levels of cAMP. Pertussis toxin catalyzes ADP-ribosylation of Gα(i), which inactivates the Gα(i) protein and attenuates inhibition of adenylate cyclase (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAdenylate cyclase-inhibiting G alpha protein; G protein subunit alpha i1; G-alpha i1; Gi; Gi1 protein alpha subunit; GNAI1; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1; Guanine nucleotide-binding protein G(i) subunit alpha-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799794798761,"sku":"5290S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5290s","provider":"Iright","version":"1.0","type":"link"}