{"product_id":"cst-52933t","title":"CST,  52933T, HRI (F9Y7H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HRI. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HRI (F9Y7H) Rabbit Monoclonal Antibody (CST #52933) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHRI (F9Y7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total HRI protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of human HRI protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHeme-regulated inhibitor (HRI), a heme-regulated eukaryotic initiation factor 2α (eIF2α) kinase, is a cytosolic protein kinase that is highly expressed in erythroid cells (1,2). Upon stress conditions such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction, and heat shock, HRI is a key activator of the integrated stress response (ISR) (3-7). Following activation, HRI is autophosphorylated, leading to eIF2α phosphorylation, which controls protein synthesis by inhibiting translational initiation (3-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nE2AK1; EIF2AK1; eukaryotic translation initiation factor 2 alpha kinase 1; Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; hHRI; HRI; KIAA1369; LEMSPAD\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 82\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799795191977,"sku":"52933T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-52933t","provider":"Iright","version":"1.0","type":"link"}