{"product_id":"cst-5335s","title":"CST,  5335S, Acetyl-alpha-Tubulin (Lys40) (D20G3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying tubulin alpha (Lys40) acetylate. Validated for WB,IP,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Acetyl-alpha-Tubulin (Lys40) (D20G3) Rabbit Monoclonal Antibody (CST #5335) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Frozen): 1:800 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAcetyl-alpha-Tubulin (Lys40) (D20G3) Rabbit Monoclonal Antibody detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Zebrafish\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α\/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis\/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). The Elongator complex catalytic subunit (Elp3) acetylates α-tubulin at Lys40 while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-transcriptional modification may be required for dynamic cell shape remodeling, cell motility, tubulin stability and terminal branching of cortical neurons (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nalpha tubulin; Alpha-tubulin ubiquitous; Detyrosinated tubulin alpha-1B chain; K-ALPHA-1; TBA1B; TUBA1B; tubulin alpha 1b; Tubulin alpha-1B chain; Tubulin alpha-ubiquitous chain; Tubulin K-alpha-1; tubulin, alpha 1b; tubulin, alpha, ubiquitous\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Z\nSENSITIVITY: Endogenous\nMW (kDa): 52\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799799156905,"sku":"5335S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5335s","provider":"Iright","version":"1.0","type":"link"}