{"product_id":"cst-53455s","title":"CST,  53455S, NSC-95397","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nNSC-95397 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.61 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles .\n\u003cb\u003eBackground\u003c\/b\u003e\nNSC-95397 is a quinone-containing inhibitor of the cdc25 dual-specificity phosphatases responsible for regulating eukaryotic cell entry into mitosis. The primary substrate of cdc25A is CDK2, which allows progression through the G1\/S and intra-S checkpoints; cdc25C activates CDK1 to overcome the G2\/M checkpoint and allow mitotic entry (1). cdc25 phosphatase inhibition by NSC-95397 varies, with dissociation constants for cdc25A, -B, and -C of Ki = 32 nM, 96 nM, and 40 nM, respectively (2). NSC-95397 treatment of cancer cell lines leads to cell growth inhibition and cell cycle arrest at G2\/M (1). Similar growth inhibition was seen in human hepatoma and breast cancer cells (3). Exposure of human erythrocytes to NSC-95397 triggers cell shrinkage, cell membrane scrambling, and eryptosis (4). The dual-specificity phosphatases MAP Kinase phosphatase 1 (MKP1) and MKP3 are likewise inhibited by NSC-95397, with NSC-95397 treatment followed by inhibited proliferation and reduced cell viability in a number of colon cancer cell lines (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2,3-bis[(2-Hydroxyethyl)thiol]-1,4-naphthoquinone","brand":"CST","offers":[{"title":"Default Title","offer_id":46800696017065,"sku":"53455S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-53455s","provider":"Iright","version":"1.0","type":"link"}