{"product_id":"cst-5379s","title":"CST,  5379S, Rictor (D16H9) Rabbit Monoclonal Antibody (Sepharose® Bead Conjugate)","description":"Monoclonal Antibody for studying Rictor. Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, Rictor (D16H9) Rabbit Monoclonal Antibody (Sepharose\u003csup\u003e®\u003c\/sup\u003e Bead Conjugate) (CST #5379) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunoprecipitation: 1:20\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRictor (D16H9) Rabbit Monoclonal Antibody (Sepharose\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Leu1121 of human rictor protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt\/PKB (2). This phosphorylation is essential for full Akt\/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; mAVO3; MGC39830; PIA; pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RICTR; RPTOR independent companion of MTOR complex 2; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800799334569,"sku":"5379S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5379s","provider":"Iright","version":"1.0","type":"link"}