{"product_id":"cst-5439s","title":"CST,  5439S, SKAR alpha\/beta (D65E8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SKAR. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SKAR alpha\/beta (D65E8) Rabbit Monoclonal Antibody (CST #5439) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSKAR alpha\/beta (D65E8) Rabbit Monoclonal Antibody detects endogenous levels of total SKAR protein, α and β isoforms. By western blot, this antibody detects an approximately 21 kDa band of unknown origin in all primate cell types tested.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human SKAR.\n\u003cb\u003eBackground\u003c\/b\u003e\np70 S6 kinase is a mitogen activated Ser\/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP\/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n46 kDa DNA polymerase delta interaction protein; DNA polymerase delta interacting protein 3; KIAA1649; p46; PDIP3; PDIP46; POLDIP3; polymerase (DNA-directed), delta interacting protein 3; polymerase (DNA) delta interacting protein 3; polymerase delta interacting protein 46; Polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly\/REF-like target; SKAR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 46, 43\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799809446057,"sku":"5439S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5439s","provider":"Iright","version":"1.0","type":"link"}