{"product_id":"cst-54551t","title":"CST,  54551T, NKX6.1 (D8O4R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NKX6-1. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, NKX6.1 (D8O4R) Rabbit Monoclonal Antibody (CST #54551) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:1600 - 1:6400\nImmunofluorescence (Immunocytochemistry): 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99540 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNKX6.1 (D8O4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total NKX6.1 protein. Note: Some non-specific staining of squamous epithelium has been observed by IHC. It is unclear if the antibody works on human samples in western blotting but is predicted to based on 100% sequence homology.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human NKX6.1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe homeodomain protein NKX6.1 is a transcription factor that regulates pancreatic β-cell development (1). Overexpressed NKX6.1 stimulates rat pancreatic β-cell proliferation and increases glucose-stimulated insulin secretion (GSIS) (2). The effect on GSIS was shown to be mediated by the upregulation of prohormone VGF expression and the subsequent potentiation by TLQP-21, a peptide derived from VGF (3). Both nuclear receptors Nr4a1 and Nr4a3 are essential for pancreatic β-cell proliferation driven by overexpressed NKX6.1 (4). In addition, studies suggest that NKX6.1 is a suppressor for epithelial-mesenchymal transition (EMT), leading to inhibition of tumor metastasis (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHomeobox protein NK-6 homolog A; Homeobox protein Nkx-6.1; NK homeo box, family 6, member A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1; NKX6-1; NKX6.1; NKX61; NKX6A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 44, 46\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799810855081,"sku":"54551T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-54551t","provider":"Iright","version":"1.0","type":"link"}