{"product_id":"cst-54618t","title":"CST,  54618T, Cytochrome b (E7F8Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MT-CYB\/Cytochrome B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome b (E7F8Z) Rabbit Monoclonal Antibody (CST #54618) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCytochrome b (E7F8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total cytochrome b protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human cytochrome b protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCytochrome b, encoded by the gene , is a subunit of ubiquinol-cytochrome c reductase, also known as cytochrome bc1, or complex III, of the mitochondrial respiratory (electron transport) chain (1). This protein complex mediates electron transfer from ubiquinol to cytochrome c, contributing to the generation of a proton gradient that powers ATP synthesis (2). Cytochrome b is an integral membrane protein and notable for being the only protein within complex III that is encoded by the mitochondrial genome. Genetic sequence analyses have identified mutations in  that are linked to a variety of diseases and pathologies (3,4). Natural variations in the sequence of  have also proven highly useful as a tool to determine phylogenetic relationships between organisms (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCOB; Complex III subunit 3; Complex III subunit III; CYB; CYTB; Cytochrome b; cytochrome b (mitochondrion); Cytochrome b-c1 complex subunit 3; mitochondrially encoded cytochrome b; MT-CYB; MTCYB; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 26\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799811575977,"sku":"54618T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-54618t","provider":"Iright","version":"1.0","type":"link"}