{"product_id":"cst-5474s","title":"CST,  5474S, Diap2 Antibody","description":"Polyclonal Antibody for studying DIAP2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Diap2 Antibody (CST #5474) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDiap2 Antibody recognizes endogenous levels of total diap2 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human diap2 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nFormins are a family of large multidomain actin nucleation\/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia\/diap) subfamily, including mDia1\/diap1, mDia2\/diap3 and mDia3\/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia\/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity and cell shape (reviewed in 1,2). mDia1\/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6). mDia3\/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7). Rho-dependent activation of mDia2\/diap3 is important in assembly of the contractile ring during cytokinesis (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDIA; DIA2; DIAP2; DIAPH2; diaphanous 2; diaphanous homolog 2; diaphanous homolog 2 (Drosophila); diaphanous related formin 2; Diaphanous-2; Diaphanous-related formin-2; diaphorase-2; DRF2; FLJ11167; mDia3; POF; POF2; POF2A; Protein diaphanous homolog 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 130\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799812526249,"sku":"5474S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5474s","provider":"Iright","version":"1.0","type":"link"}