{"product_id":"cst-5480s","title":"CST,  5480S, CHAF1A (D77D5) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CHAF1A. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CHAF1A (D77D5) Rabbit Monoclonal Antibody (CST #5480) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCHAF1A (D77D5) Rabbit Monoclonal Antibody detects endogenous levels of total CHAF1A protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro303 of human CHAF1A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nChromatin assembly factor 1 (CAF-1) is a histone H3\/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3\/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A\/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3\/H4 tetramers onto replicating DNA (2-6). Assembly of histone H2A\/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAF; CAF-1; CAF-1 subunit A; CAF-1A; CAF-I 150 kDa subunit; CAF-I p150; CAF1; CAF1A; CAF1B; CAF1P150; CHAF1A; Chromatin assembly factor 1 subunit A; chromatin assembly factor 1, subunit A (p150); chromatin assembly factor I (150 kDa); Chromatin assembly factor I p150 subunit; CTB-50L17.7; hp150; MGC71229; P150\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 145\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799812821161,"sku":"5480S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5480s","provider":"Iright","version":"1.0","type":"link"}