{"product_id":"cst-54939t","title":"CST,  54939T, TIFA (E9W1L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TIFA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TIFA (E9W1L) Rabbit Monoclonal Antibody (CST #54939) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTIFA (E9W1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIFA protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TIFA protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTRAF-interacting protein with FHA domain-containing protein A (TIFA) is a cytosolic adaptor protein that is a critical node in an innate immune signaling axis that is activated upon detection of proinflammatory stimuli derived from a bacterial pathogen-associated molecular pattern metabolite, ADP-heptose (1-4). ADP-heptose binds and activates Alpha-protein kinase 1, which drives the phosphorylation-dependent oligomerization and activation of TIFA (5,6). Activated TIFA is then poised to shape the innate immune response by triggering TRAF6 oligomerization, IKK activation, and NF-κB-dependent proinflammatory gene transcription (1,2,5,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nMGC20791; Putative MAPK-activating protein PM14; Putative NF-kappa-B-activating protein 20; T2BP; T6BP; TIFA; TIFAA; TRAF interacting protein with forkhead associated domain; TRAF-interacting protein with a forkhead-associated domain; TRAF-interacting protein with FHA domain-containing protein A; TRAF-interacting protein with forkhead-associated domain; TRAF2 binding protein; TRAF2-binding protein; TRAF6 binding protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 21\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799814262953,"sku":"54939T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-54939t","provider":"Iright","version":"1.0","type":"link"}