{"product_id":"cst-54979t","title":"CST,  54979T, VISTA (D5L5T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying VISTA mouse. Validated for WB,IP,IHC,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, VISTA (D5L5T) Rabbit Monoclonal Antibody (CST #54979) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nIHC Leica Bond: 1:300\nImmunohistochemistry (Paraffin): 1:300\nImmunofluorescence (Frozen): 1:200\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56548 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVISTA (D5L5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total VISTA protein. This antibody is not recommended for immunohistochemical analysis of human tissues. Instead, VISTA (D1L2G) Rabbit Monoclonal Antibody #64953 is suggested.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val291 of mouse VISTA protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nVISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n4632428N05Rik; Die; Dies1; differentiation of ESCs 1; GI24; Gi24 receptor; PD-1; PD-1H; Platelet receptor Gi24; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation; VISTA; Vsir\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 45-65\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799814394025,"sku":"54979T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-54979t","provider":"Iright","version":"1.0","type":"link"}