{"product_id":"cst-5524s","title":"CST,  5524S, PITSLRE\/CDK11 (D88B3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PITSLRE. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PITSLRE\/CDK11 (D88B3) Rabbit Monoclonal Antibody (CST #5524) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPITSLRE\/CDK11 (D88B3) Rabbit Monoclonal Antibody recognizes endogenous levels of total PITSLRE\/CDK11 protein. This antibody will detect the full-length (CDK11\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met734 of human PITSLRE\/CDK11 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPITSLRE, alternatively known as cell division kinase 11 (CDK11), is the result of duplication of the gene (1). and encode nearly identical serine\/threonine protein kinases, PITSLREB and PITSLREA respectively, both belonging to the p34 family of protein kinases (2). Full-length PITSLRE\/CDK11 (commonly referred to as CDK11 ) is expressed ubiquitously throughout the cell cycle whereas a smaller, alternate transcript (CDK11 ), the result of internal ribosomal entry, is expressed only during the G2\/M transition where it promotes centrosome maturation and spindle formation (3-5). During induction of apoptosis by Fas or TNF, or anoikis, PITSLRE\/CDK11 is cleaved by caspases to generate p110C, an approximately 46 kDa protein that contains the catalytically active kinase domain of PITSLRE\/CDK11 that interacts with and inhibits p21-activated kinase (PAK1) activity (6-8). Full length PITSLRE\/CDK11 (CDK11 ) appears to participate in pre-mRNA splicing events. This is demonstrated by the observation that CDK11 interacts with numerous splicing factors including RNPS1, 9G8\/SRSF7 and cyclin L and that CDK11 can phosphorylate and inhibit the splicing activity of 9G8\/SRSF7 (9-11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD11B; CDC-related protein kinase p58; CDC2L1; CDC2L2; CDK11; CDK11-p110; CDK11-p46; CDK11-p58; CDK11B; CDK11p58; cell division cycle 2-like 1 (PITSLRE proteins); cell division cycle 2-like 2 (PITSLRE proteins); cell division cycle 2-like protein kinase 1; cell division protein kinase 11B; CLK-1; cyclin dependent kinase 11B; Cyclin-dependent kinase 11B; FLJ59152; galactosyltransferase-associated protein kinase p58\/GTA; p58; p58 CLK-1; p58CDC2L1; p58CLK-1; PITSLRE; PITSLRE serine\/threonine-protein kinase CDC2L1; PITSLREA; PK58\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799816065193,"sku":"5524S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5524s","provider":"Iright","version":"1.0","type":"link"}