{"product_id":"cst-5536l","title":"CST,  5536L, Phospho-mTOR (Ser2448) (D9C2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for WB,WB,WB,IP,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-mTOR (Ser2448) (D9C2) Rabbit Monoclonal Antibody (CST #5536) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFluorescent Western: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 78080 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-mTOR (Ser2448) (D9C2) Rabbit Monoclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at Ser2448.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser\/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase\/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine\/threonine kinase); mechanistic target of rapamycin kinase; mTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine\/threonine-protein kinase mTOR; SKS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 289\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799816786089,"sku":"5536L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5536l","provider":"Iright","version":"1.0","type":"link"}