{"product_id":"cst-55494s","title":"CST,  55494S, GFP (5G4) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying GFP jellyfish. Validated for Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GFP (5G4) Mouse Monoclonal Antibody (CST #55494) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGFP (5G4) Mouse Monoclonal Antibody detects native GFP and YFP-tagged proteins exogenously expressed in cells, but does not detect denatured GFP. This antibody does not detect RFP-tagged proteins. Please note that the GFP and YFP tags add approximately 27 kDa to the molecular weight of the fusion protein.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with native GFP.\n\u003cb\u003eBackground\u003c\/b\u003e\nEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nGFP; Green fluorescent protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Mouse IgG2b","brand":"CST","offers":[{"title":"Default Title","offer_id":46800248209577,"sku":"55494S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-55494s","provider":"Iright","version":"1.0","type":"link"}