{"product_id":"cst-55506l","title":"CST,  55506L, Cyclin D1 (E3P5S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Cyclin D1. Validated for WB,IP,IHC,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Cyclin D1 (E3P5S) Rabbit Monoclonal Antibody (CST #55506) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nIHC Leica Bond: 1:250 - 1:1000\nImmunohistochemistry (Paraffin): 1:250 - 1:1000\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 66467 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCyclin D1 (E3P5S) Rabbit Monoclonal Antibody recognizes endogenous levels of total cyclin D1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala284 of human cyclin D1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nActivity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D\/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D\/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-cell CLL\/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; BCL1; CCND1; CycD1; cyclin D1; D11S287E; G1\/S-specific cyclin D1; G1\/S-specific cyclin-D1; PRAD1; PRAD1 oncogene; U21B31\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 36\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799818096809,"sku":"55506L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-55506l","provider":"Iright","version":"1.0","type":"link"}