{"product_id":"cst-5560s","title":"CST,  5560S, DJ-1 (D21E11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DJ-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DJ-1 (D21E11) Rabbit Monoclonal Antibody (CST #5560) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDJ-1 (D21E11) Rabbit Monoclonal Antibody detects endogenous levels of total DJ-1 protein. This antibody is recommended to detect human samples but may also detect mouse and rat samples weakly.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly150 of human DJ-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nParkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-\/-) mice, resulting in reduced long-term depression (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDJ-1; DJ1; epididymis secretory sperm binding protein Li 67p; FLJ27376; FLJ34360; FLJ92274; GATD2; HEL-S-67p; Maillard deglycase; Oncogene DJ1; PARK7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; parkinson protein 7; Parkinsonism associated deglycase; Parkinsonism-associated deglycase; protein deglycase DJ-1; Protein DJ-1; Protein\/nucleic acid deglycase DJ-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 22\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799819210921,"sku":"5560S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5560s","provider":"Iright","version":"1.0","type":"link"}