{"product_id":"cst-5567s","title":"CST,  5567S, PTMScanÂ® Phospho-T*PP Motif (T*PP) Rabbit Monoclonal Antibody Kit","description":"PTMScan for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan Â® Motif antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS\/MS analysis. CST recommends the use of PTMScan Â® IAP Buffer #9993 included in the kit.\n\u003cb\u003eStorage\u003c\/b\u003e\nAntibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: PTMScan\n\u003cb\u003eBackground\u003c\/b\u003e\nA T\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nmotif; phospho-threonine; threonine","brand":"CST","offers":[{"title":"Default Title","offer_id":46800795762857,"sku":"5567S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5567s","provider":"Iright","version":"1.0","type":"link"}