{"product_id":"cst-55728t","title":"CST,  55728T, PCSK9 (D5K4S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PCSK9. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PCSK9 (D5K4S) Rabbit Monoclonal Antibody (CST #55728) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPCSK9 (D5K4S) Rabbit Monoclonal Antibody recognizes endogenous levels of total PCSK9 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PCSK9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1\/3, PC2, PC4, PACE4, PC5\/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3). Mutations in the PCSK9 gene (encoding proprotein converts subtilisin\/kexin type 9, PC9) have been found to cause autosomal-dominant hypercholesterolemia. PCSK9 was therefore demonstrated to be a key factor involved in lipoprotein metabolism (4). PCSK9 acts as a chaperone protein that binds the LDL receptor (LDLR) at the cell membrane and induces LDLR lysosomal degradation rather than recycling (5). PCSK9 inhibition has since been a new therapeutic strategy for the treatment of hypercholesterolemia (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nconvertase subtilisin\/kexin type 9 preproprotein; FH3; FHCL3; HCHOLA3; LDLCQ1; NARC-1; NARC1; neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein convertase 9; Proprotein convertase subtilisin\/kexin type 9; Subtilisin\/kexin-like protease PC9\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 65, 80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799819899049,"sku":"55728T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-55728t","provider":"Iright","version":"1.0","type":"link"}