{"product_id":"cst-5593s","title":"CST,  5593S, Troponin T (Cardiac) Antibody","description":"Polyclonal Antibody for studying TNNT2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Troponin T (Cardiac) Antibody (CST #5593) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTroponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein.\nSpecies Reactivity: Human, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro69 of human cardiac Troponin T protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nTroponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca concentration. Troponin consists of three subunits: the Ca -binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca sensitivity by reducing the Ca binding affinity of TnC (1-3). The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT\/TNNT1), fast skeletal muscle (fsTnT\/TNNT3) and in cardiac muscle (cTnT\/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCardiac muscle troponin T; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; RCM3; TNNT2; TnTc; troponin T type 2 (cardiac); troponin T type 2, cardiac; Troponin T, cardiac muscle; troponin T2, cardiac; troponin T2, cardiac type; truncated cardiac troponin T\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799821340841,"sku":"5593S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5593s","provider":"Iright","version":"1.0","type":"link"}