{"product_id":"cst-56077s","title":"CST,  56077S, Phospho-IRAK4 (Thr345\/Ser346) (F7B4D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IRAK4 (Thr345\/Ser346) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-IRAK4 (Thr345\/Ser346) (F7B4D) Rabbit Monoclonal Antibody (CST #56077) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-IRAK4 (Thr345\/Ser346) (F7B4D) Rabbit Monoclonal Antibody recognizes endogenous levels of IRAK4 protein only when phosphorylated at Thr345 and Ser346.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr345\/Ser346 of human IRAK4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine\/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3\/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3). Upon IL-1 receptor (IL-1R) and toll-like receptor (TLR) ligation, IRAK1 and IRAK4 are rapidly recruited to the receptor by the adapter MyD88 (4). IRAK1 is phosphorylated by IRAK4 at Thr209 and Thr387 (5), followed by sequential auto-hyperphosphorylation in various domains.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nIMD67; interleukin 1 receptor associated kinase 4; Interleukin-1 receptor-associated kinase 4; IPD1; IRAK-4; IRAK4; NY-REN-64; Q8TDF7; REN64; Renal carcinoma antigen NY-REN-64\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799822684329,"sku":"56077S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-56077s","provider":"Iright","version":"1.0","type":"link"}