{"product_id":"cst-56183s","title":"CST,  56183S, Firefly Luciferase (E9W9V) Feline Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying luciferase firefly. Validated for Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Firefly Luciferase (E9W9V) Feline Chimeric Monoclonal Antibody (CST #56183) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFirefly Luciferase (E9W9V) Feline Chimeric Monoclonal Antibody recognizes exogenously expressed proteins containing firefly luciferase (\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from Firefly Luciferase (E9W9V) Rabbit mAb #25039 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Firefly Luciferase (E9W9V) Rabbit mAb #25039, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase ( ) protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLuciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly, , that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg ) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase ( ) protein or its enzymatic activity (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nluciferin 4-monooxygenase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Feline chimeric IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800268951721,"sku":"56183S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-56183s","provider":"Iright","version":"1.0","type":"link"}