{"product_id":"cst-56191t","title":"CST,  56191T, ELOF1 (F6D3C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ELOF1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ELOF1 (F6D3C) Rabbit Monoclonal Antibody (CST #56191) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nELOF1 (F6D3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELOF1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val37 of human ELOF1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDNA lesions in the template strand of actively transcribed genes result in genome-wide transcriptional arrest. The transcription-coupled nucleotide excision repair (TC-NER) pathway efficiently eliminates transcription-blocking DNA lesions (TBLs) (1). ELOF1 is a component of the transcriptional elongating Pol II complex (2). The protein ensures high-speed elongation processivity during mRNA transcription on the DNA template (3). ELOF1 is an important player of the TC-NER-complex (4). ELOF1 facilitates Pol II ubiquitylation and ensures correct TC-NER-complex assembly upon DNA damage (5). The protein promotes UV-stimulated scaffold protein A (UVSSA) and lesion-stalled Pol II binding, resulting in subsequent transcription factor IIH (TFIIH) recruitment, which is crucial in the full incision complex assembly to excise the TBL and restart transcription (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nELF1; ELF1 homolog, elongation factor 1; ELOF1; elongation factor 1 homolog; elongation factor 1 homolog (ELF1, S. cerevisiae); elongation factor 1 homolog (S. cerevisiae); Transcription elongation factor 1 homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 14\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799823470761,"sku":"56191T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-56191t","provider":"Iright","version":"1.0","type":"link"}