{"product_id":"cst-5664s","title":"CST,  5664S, CNPase (D83E10) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CNPase. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CNPase (D83E10) Rabbit Monoclonal Antibody (CST #5664) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunofluorescence (Frozen): 1:50 - 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51765 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCNPase (D83E10) Rabbit Monoclonal Antibody recognizes endogenous levels of total CNPase protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val81 of human CNPase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) catalyzes the hydrolysis of 2', 3'-cyclic nucleotides to produce 2'-nucleotides. The molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2', 3' cyclic nucleotide 3' phosphohydrolase; 2',3'-cyclic nucleotide 3' phosphodiesterase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CN37; CNP; CNP1; CNPase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 47\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799827599529,"sku":"5664S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5664s","provider":"Iright","version":"1.0","type":"link"}