{"product_id":"cst-56678s","title":"CST,  56678S, GPR75 (F3P5Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GPR75. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GPR75 (F3P5Y) Rabbit Monoclonal Antibody (CST #56678) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGPR75 (F3P5Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPR75 protein. This antibody detects a 50 kDa protein of unknown identity in some tissues.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala430 of human GPR75 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGPR75 is a G protein-coupled receptor. Protein-truncating variants were associated with leanness and protection against obesity in humans. In addition, studies show that knockout (KO) mice fed with a high-fat diet are resistant to weight gain and have improved glycemic control and insulin sensitivity compared with wild-type (WT) mice (1). Furthermore, studies show that, in mice, mRNA expression is the most abundant in the brain. is widely expressed in the mouse brain, and a subset of is co-expressed with appetite-regulating hypothalamic neurons. KO mice are protected against liver lipid accumulation induced by a high-fat diet compared with WT mice (2). Moreover, studies on KO mice suggest a role of Gpr75 in the development of hypoxia-induced pulmonary hypertension (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nG protein-coupled receptor 75; GPR75; GPRchr2; MGC132002; Probable G-protein coupled receptor 75; WI31133\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 75\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799828058281,"sku":"56678S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-56678s","provider":"Iright","version":"1.0","type":"link"}