{"product_id":"cst-56719t","title":"CST,  56719T, NALP1 (E3F2U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NALP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NALP1 (E3F2U) Rabbit Monoclonal Antibody (CST #56719) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNALP1 (E3F2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total NALP1 protein. This antibody recognizes both the 165 kDa full-length NALP1 and the auto-processed 27 kDa C-terminal fragment.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NALP1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNALP1 (DEFCAP\/NAC\/CARD7) is an NLR (Nod-like receptor) family member that has been implicated in the regulation of apoptosis and inflammatory responses (1-5). Structurally, NALP contains an amino-terminal PYRIN domain, followed by a nucleotide-binding site (NBS), a leucine-rich repeat region (LRR), and a carboxy-terminal CARD domain. NALP1 interacts strongly with caspase-2 and weakly with caspase-9, and induces apoptosis when overexpressed (3). Similar to a related Ced-4 family member Apaf-1, it was also shown to be involved in cytochrome c-dependent caspase activation (2). It has also been shown to be part of the \"inflammasome\" comprised of caspase-1, caspase-5, and Pycard\/ASC, which is critical in the processing of pro-inflammatory cytokines like IL-1β (6). Two major isoforms were identified for NALP1, which differ in a 44 amino acid region within the LRR (3). In addition, like NALP3, a short NALP1 isoform lacking the LRR (NALP1 ) likely exists (7). Polymorphisms in NALP1 have been associated with autoimmune diseases (8) and susceptibility to toxins (9). NALP1 is autoproteolytically processed into a large N-terminal and a small C-terminal fragment, which are non-covalently associated. Upon activation, the C-terminal is released and forms inflammasomes with other proteins (10-12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIADK; CARD7; caspase recruitment domain protein 7; Caspase recruitment domain-containing protein 7; CIDED; CLR17.1; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAP-L\/S; DKFZp586O1822; JRRP; KIAA0926; MSPC; NAC; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1; NALP1; NLR family pyrin domain containing 1; NLR family, pyrin domain containing 1; NLRP1; Nucleotide-binding domain and caspase recruitment domain; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1; PP1044; SLEV1; VAMAS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 165\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799828648105,"sku":"56719T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-56719t","provider":"Iright","version":"1.0","type":"link"}