{"product_id":"cst-5681s","title":"CST,  5681S, IWS1 Antibody","description":"Polyclonal Antibody for studying IWS1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, IWS1 Antibody (CST #5681) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIWS1 Antibody detects endogenous levels of total IWS1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IWS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nVarious steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation (1,2). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps (1,2). IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone (1,2). IWS1 also recruits another CTD-binding protein, HYPB\/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation (2). Thus, IWS1 links Spt6 and HYPB\/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp761G0123; FLJ10006; FLJ14655; FLJ32319; interacts with Spt6; interacts with SUPT6H, CTD assembly factor 1; IWS1; IWS1 homolog; IWS1 homolog (S. cerevisiae); IWS1-like protein; IWS1, SUPT6H interacting protein; IWS1L; MGC126375; MGC126376; Protein IWS1 homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799829336233,"sku":"5681S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5681s","provider":"Iright","version":"1.0","type":"link"}