{"product_id":"cst-5683s","title":"CST,  5683S, PERK (D11A8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PERK. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PERK (D11A8) Rabbit Monoclonal Antibody (CST #5683) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:1000 - 1:4000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPERK (D11A8) Rabbit Monoclonal Antibody recognizes endogenous levels of total PERK protein. Staining of red blood cells has been observed. The specificity of this staining is unknown.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp164 of human PERK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp781H1925; E2AK3; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; heme-regulated EIF2-alpha kinase; HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PERK; PRKR-like endoplasmic reticulum kinase; truncated eukaryotic translation initiation factor 2 alpha kinase 3; WRS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799829500073,"sku":"5683S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5683s","provider":"Iright","version":"1.0","type":"link"}