{"product_id":"cst-56853sf","title":"CST,  56853SF, TCF1\/TCF7 (E6O1K) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying TCF7 mouse. Validated for WB,IHC,IF,IF,F. Highly specific and rigorously validated in-house, TCF1\/TCF7 (E6O1K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #56853) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #16551. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 16551 .\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTCF1\/TCF7 (E6O1K) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total TCF1\/TCF7 proteins. This antibody should detect all TCF1\/TCF7 isoforms, including those lacking the amino-terminal β-catenin binding domain. This antibody does not cross-react with LEF1, TCF3\/TCF7L1, or TCF4\/TCF7L2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse TCF1\/TCF7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1\/TCF7), TCF3\/TCF7L1, and TCF4\/TCF7L2 (1). LEF1 and TCF1\/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5). TCF1\/TCF7 has several isoforms due to alternative splicing and transcription from an alternative promoter. The isoforms generated by the alternative promoter do not contain the amino-terminal β-catenin binding domain and therefore may function in a dominant negative manner (6). TCF1\/TCF7 displays dynamic expression both in the total amount and the type of isoforms expressed in T cells during development and differentiation (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI465550; OTTMUSP00000005800; OTTMUSP00000005801; T cell factor-1; T-cell factor 1; T-cell-specific transcription factor 1; TCF-1; TCF-7; Tcf1; Tcf7; Transcription factor 7; transcription factor 7, T cell specific; transcription factor 7, T-cell specific\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28-50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800990535849,"sku":"56853SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-56853sf","provider":"Iright","version":"1.0","type":"link"}