{"product_id":"cst-5691s","title":"CST,  5691S, LCMT1 (4A4) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying LCMT1 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, LCMT1 (4A4) Mouse Monoclonal Antibody (CST #5691) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLCMT1 (4A4) Mouse Monoclonal Antibody recognizes endogenous levels of total LCMT1 protein.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein specific to mouse LCMT1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLeucine carboxyl methyltransferase-1 (LCMT1) is an important mammalian methyltransferase that methylates the catalytic C subunit of protein phosphatase 2A (PP2A) at Leu309 (1,2). X-ray crystallography analysis reveals that the structural integrity of human LCMT1 and cofactor S-adenosylmethionine complex enables proper interaction with PP2A (3). Evidence suggests that functional LCMT1 is crucial for the ability of PP2A to form heterotrimers with the B\/B55 and Bα family of regulatory subunits (4,5). Impaired LCMT1 activity is shown to disrupt mitosis and cell survival (5). In neurodegenerative models, increases in endogenous levels of LCMT1, methylated PP2A (C), and Bα induce the development and differentiation of neuroblastoma cells (5-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAL033290; Lcm; Lcmt; LCMT-; LCMT-1; Lcmt1; Leucine carboxyl methyltransferase 1; Leucine carboxyl methyltransferase 1, isoform CRA_b; MGC11807\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 39\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800103997609,"sku":"5691S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5691s","provider":"Iright","version":"1.0","type":"link"}