{"product_id":"cst-57219sf","title":"CST,  57219SF, CCL5\/RANTES (E9S2K) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying CCL5. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CCL5\/RANTES (E9S2K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #57219) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier-free version of product #36467. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN, or CUT\u0026amp;Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 36467 .\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCCL5\/RANTES (E9S2K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CCL5\/RANTES protein. This antibody recognizes human CCL5\/RANTES protein and is also reactive with mouse CCL5\/RANTES; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Non-specific staining was observed in human pancreatic islets by immunohistochemistry.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with human CCL5\/RANTES recombinant protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nRANTES\/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the C-C or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8 T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nbeta-chemokine RANTES; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; Eosinophil chemotactic cytokine; MGC17164; RANTES; RANTES(3-68); RANTES(4-68); regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SIS-delta; SISd; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 10\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800992829609,"sku":"57219SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-57219sf","provider":"Iright","version":"1.0","type":"link"}