{"product_id":"cst-5724s","title":"CST,  5724S, Phospho-Axl (Tyr702) (D12B2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Axl (Tyr702) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Axl (Tyr702) (D12B2) Rabbit Monoclonal Antibody (CST #5724) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28451 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Axl (Tyr702) (D12B2) Rabbit Monoclonal Antibody detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAxl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6\/Axl signaling in cell growth and survival in normal and cancer cells (4). Phosphorylation of Axl Receptor on Tyr702 was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS\/MS platform for phosphorylation site discovery (5). Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARK; AXL; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence\/gene; JTK11; oncogene AXL; Tyro7; Tyrosine-protein kinase receptor UFO; UFO\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 138\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800992862377,"sku":"5724S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5724s","provider":"Iright","version":"1.0","type":"link"}