{"product_id":"cst-57497s","title":"CST,  57497S, LEF1 (C12A5) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate)","description":"Monoclonal Antibody for studying LEF1. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, LEF1 (C12A5) Rabbit Monoclonal Antibody (Pacific Blue\u003csup\u003e™\u003c\/sup\u003e Conjugate) (CST #57497) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLEF1 (C12A5) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate) detects endogenous levels of total LEF1 protein. This antibody conjugate does not recognize the dominant negative forms of LEF1 generated by an alternative promoter.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro82 of human LEF1.\n\u003cb\u003eBackground\u003c\/b\u003e\nLEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1\/TCF7), TCF3\/TCF7L1, and TCF4\/TCF7L2 (1). LEF1 and TCF1\/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5). LEF1 has several isoforms due to alternative splicing. LEF1 also has an alternative promoter that is preferentially active in lymphocytes. The isoforms generated by this alternative promoter have no amino-terminal β-catenin binding domain and may function in a dominant negative manner (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp586H0919; FLJ46390; LEF-1; LEF1; lymphoid enhancer binding factor 1; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; TCF10; TCF1ALPHA; TCF7L3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800448913577,"sku":"57497S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-57497s","provider":"Iright","version":"1.0","type":"link"}