{"product_id":"cst-57625s","title":"CST,  57625S, p300 (E8S2V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying p300 mouse. Validated for WB,WB,IP,ChIP,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, p300 (E8S2V) Rabbit Monoclonal Antibody (CST #57625) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:200\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np300 (E8S2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total p300 protein. This antibody may detect a non-specific band of unknown identity at 200 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse p300 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP\/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C\/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP\/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nA430090G16; A730011L11; E1A binding protein p300; E1A-associated protein p300; Ep300; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; KAT3; KAT3B; p30; p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein propionyltransferase p300\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 300\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800997318825,"sku":"57625S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-57625s","provider":"Iright","version":"1.0","type":"link"}