{"product_id":"cst-57921t","title":"CST,  57921T, HIF-2 alpha (E2N9W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HIF2A mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-2 alpha (E2N9W) Rabbit Monoclonal Antibody (CST #57921) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHIF-2 alpha (E2N9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total HIF-2α protein. This antibody does not cross-react with HIF-1α protein.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse HIF-2α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin\/proteasome pathway (1,2). Hypoxia inhibits degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nbHLHe7; bHLHe73; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; Epas1; HIF; Hif like protein; HIF-1-alpha-like factor; HIF-2; HIF-2-alpha; HIF-2alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2-alpha; Hif2a; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible transcription factor 2alpha; Hypoxia-inducible factor 2-alpha; mHLF; MOP2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 120\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800104849577,"sku":"57921T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-57921t","provider":"Iright","version":"1.0","type":"link"}