{"product_id":"cst-58036l","title":"CST,  58036L, Protein L (PE Conjugate)","description":"Miscellaneous for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nRecommended Dilution: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nProtein L (PE Conjugate) is recombinantly derived and genetically engineered such as to remove the cell wall-, cell membrane-, and albumin-binding regions of Protein L to enhance binding to immunoglobulins. Conjugation to PE makes this product ideally suited for direct flow cytometric analysis of cells expressing κ light chain-containing immunoglobulins as well as immunoglobulin fragments that contain κ light chains, such as scFvs.\nSpecies Reactivity: Human, Mouse, Rat, Pig\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein L was first isolated from the surface of bacterial species (1). Unlike Protein A and Protein G that differentially bind to the Fc region of immunoglobulin isotype heavy chains, Protein L contains Ig binding domains that interact with the κ light chain variable domain without disrupting paratope function (2-4). This attribute not only facilitates binding to a broader range of immunoglobulin classes and subclasses than either Protein A or Protein G but also allows for binding to Fab fragments and single chain variable fragments (scFvs) that contain κ light chain sequences. Protein L does, however, display specificity with respect to κ light chain subtypes. In human, Protein L binds to VκI, VκIII, and VκIV subtypes whereas binding is restricted to VκI in mice (4).","brand":"CST","offers":[{"title":"Default Title","offer_id":46801056366761,"sku":"58036L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58036l","provider":"Iright","version":"1.0","type":"link"}