{"product_id":"cst-58118t","title":"CST,  58118T, NLRP4 (F7T1G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NALP4. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP4 (F7T1G) Rabbit Monoclonal Antibody (CST #58118) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNLRP4 (F7T1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP4 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu814 of human NLRP4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNOD-like receptor (NLR) family pyrin domain containing 4 (NLRP4), also known as NALP4, is a cytosolic protein with roles in regulating both autophagy, through interactions with beclin-1, and the type I interferon response by targeting TBK1 for degradation (1,2). NLRP4 also serves as an intracellular sensor for microbes and it is recruited to phagosomes containing group A where it associates with Rho GTPases and Rho GDP dissociation inhibitor alpha (ARHGDIA) to promote xenophagy (3). NLRP4 may also play a role in cancer, as non-small cell lung cancer patients with mutations displayed improved response to immune checkpoint blockade (4). In mice, knockdown led to enhanced type I interferon production and slower tumor growth (4). Conversely, upregulation enhances DNA damage repair in pancreatic cancer cell lines, and higher expression negatively correlates with mouse BxPC-3 xenograft response to olaparib treatment (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCancer\/testis antigen 58; CLR19.5; CT58; FLJ32126; NACHT, leucine rich repeat and PYD containing 4; NACHT, LRR and PYD containing protein 4; NACHT, LRR and PYD domains-containing protein 4; NALP4; NLR family pyrin domain containing 4; NLR family, pyrin domain containing 4; NLRP4; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 4; PAAD and NACHT-containing protein 2; PAN2; PYPAF4; PYRIN and NACHT-containing protein 2; PYRIN-containing APAF1-like protein 4; Ribonuclease inhibitor 2; RNH2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 113\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800999186601,"sku":"58118T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58118t","provider":"Iright","version":"1.0","type":"link"}