{"product_id":"cst-58145s","title":"CST,  58145S, CD10\/Neprilysin (F4P6H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD10 mouse. Validated for WB,WB,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD10\/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (CST #58145) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunohistochemistry (Paraffin): 1:50\nImmunofluorescence (Frozen): 1:200 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67330 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD10\/Neprilysin (F4P6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD10\/Neprilysin protein.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10\/Neprilysin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10 GPR77 carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; common acute lymphoblastic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; Mme; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800105373865,"sku":"58145S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58145s","provider":"Iright","version":"1.0","type":"link"}