{"product_id":"cst-58208t","title":"CST,  58208T, Caspase-12 (E9T3W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Casp12 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-12 (E9T3W) Rabbit Monoclonal Antibody (CST #58208) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCaspase-12 (E9T3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Caspase-12 protein. This antibody detects the large subunit of Caspase-12 associated with activation. Bands of unknown identity were observed at 42 and 30 kDa. These may represent additional caspase-12 isoforms.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to a region within the large subunit of mouse Caspase-12 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER stress-induced apoptosis, such as high calcium concentration, low oxygen, and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain-mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCASP-12; Casp12; caspase 12; Caspase-12; caspase12; CASPC\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 55, 38, 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800105537705,"sku":"58208T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58208t","provider":"Iright","version":"1.0","type":"link"}