{"product_id":"cst-58309t","title":"CST,  58309T, DDRGK1 (F6M1S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DDRGK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DDRGK1 (F6M1S) Rabbit Monoclonal Antibody (CST #58309) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDDRGK1 (F6M1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDRGK1 protein. Based on epitope mapping, this antibody is predicted to bind isoform 1 and not isoform 2 of DDRGK1.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp244 of human DDRGK1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4). DDRGK1 (also called UFBP1, C20orf116, and Dashurin) is an ER protein that is a key component of the UFMylation system and regulator of ER homeostasis (15,16). It is also a regulator of NF-κB activity (17,18). DDRGK1 is indispensable for embryonic development and hematopoiesis (17).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC20orf116; Dashurin; DDRGK; DDRGK domain containing 1; DDRGK domain-containing protein 1; DDRGK1; dJ1187M17.3; MGC2592; SEMDSH; UFBP1; UFM1-binding and PCI domain-containing protein 1; UFM1-binding protein 1 containing a PCI domain\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801000562857,"sku":"58309T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58309t","provider":"Iright","version":"1.0","type":"link"}