{"product_id":"cst-58410s","title":"CST,  58410S, Iba1\/AIF-1 (E4O4W) Mouse Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying AIF1\/Iba1. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Iba1\/AIF-1 (E4O4W) Mouse Chimeric Monoclonal Antibody (CST #58410) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIba1\/AIF-1 (E4O4W) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total Iba1\/AIF-1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\n\u003cb\u003eBackground\u003c\/b\u003e\nIonized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1\/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1\/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1\/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial\/macrophage states (5). Iba1\/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia\/macrophages in the brain and other tissue (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse chimeric IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800296542377,"sku":"58410S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58410s","provider":"Iright","version":"1.0","type":"link"}