{"product_id":"cst-5851t","title":"CST,  5851T, GATA-6 (D61E4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GATA6. Validated for WB,WB,IHC,IF,F,ChIP,ChIP,C\u0026amp;R,C\u0026amp;T. Available in 2 sizes. Highly specific and rigorously validated in-house, GATA-6 (D61E4) Rabbit Monoclonal Antibody (CST #5851) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunohistochemistry (Paraffin): 1:100 - 1:400\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\nCUT\u0026amp;RUN: 1:50\nCUT\u0026amp;Tag: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 48888 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGATA-6 (D61E4) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATA-6 protein. Non-specific cytoplasmic staining of limited immune cells in mouse small intestine, thymus and spleen was observed by immunohistochemistry.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-6 plays a critical role in endoderm development (4). It is essential for development of the heart, gut, and other organs (5,6). Knock out of GATA-6 is embryonic lethal due to defects in formation of the heart tube and a failure to develop extraembryonic endoderm (4). Loss of expression, or loss of nuclear localization of GATA-6 is apparent in a large number of ovarian tumors (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nGATA binding protein 6; GATA-binding factor 6; GATA-binding protein 6; GATA6; Transcription factor GATA-6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801002430633,"sku":"5851T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5851t","provider":"Iright","version":"1.0","type":"link"}