{"product_id":"cst-5852t","title":"CST,  5852T, GATA-3 (D13C9) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GATA3. Validated for WB,WB,IHC,IF,F,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, GATA-3 (D13C9) Rabbit Monoclonal Antibody (CST #5852) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunohistochemistry (Paraffin): 1:1600 - 1:6400\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\nChromatin IP: 1:100\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGATA-3 (D13C9) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATA-3 protein. This antibody is not recommended for immunofluorescence analysis of frozen mouse tissues due to non-specific staining.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr63 of human GATA-3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nGATA binding protein 3; GATA-binding factor 3; GATA3; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801002496169,"sku":"5852T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-5852t","provider":"Iright","version":"1.0","type":"link"}