{"product_id":"cst-58982s","title":"CST,  58982S, CENP-F (D6X4L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CENPF. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, CENP-F (D6X4L) Rabbit Monoclonal Antibody (CST #58982) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57905 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCENP-F (D6X4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CENP-F protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1794 of human CENP-F protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2\/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; CENPF; Centromere protein F; centromere protein F, 350\/400ka (mitosin); centromere protein F, 350\/400kDa; CILD31; hcp-1; Kinetochore protein CENPF; Mitosin; PRO1779; STROMS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 450\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801005904041,"sku":"58982S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-58982s","provider":"Iright","version":"1.0","type":"link"}