{"product_id":"cst-59278s","title":"CST,  59278S, DDX21 (E9O2Q) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DDX21. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DDX21 (E9O2Q) Rabbit Monoclonal Antibody (CST #59278) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDDX21 (E9O2Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX21 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala780 of human DDX21 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDDX21, also known as RNA helicase II\/Gu, is an RNA helicase that plays important roles in transcription and ribosomal RNA (rRNA) processing (1-3). DDX21 associates with many RNA Polymerase I and II genes, including the direct binding to the rDNA locus to promote ribosome biogenesis. The helicase activity of DDX21 contributes to transcription by helping release P-TEFb from the 7SK small nuclear ribonucleoprotein (snRNP) complex (3). Nucleolar localization of DDX21 has been shown to be regulated through phosphorylation by JNK and interaction with c-Jun. Depletion of either c-Jun or JNK has shown to impair rRNA processing (4,5). DDX21 can also become acetylated by CBP, which reduces its activity. Deacetylation by SIRT7 increases DDX21 helicase activity to prevent R-loop accumulation and protect genomic stability (6). DDX21 can also form a complex with DDX1, DHX36, and TRIF to sense dsRNA, which in turn activates interferon response in innate immunity (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDDX21; DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD box protein 21; DEAD-box helicase 21; DEAD\/H (Asp-Glu-Ala-Asp\/His) box polypeptide 21; DExD-box helicase 21; DKFZp686F21172; Gu protein; Gu-alpha; GUA; GURDB; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II\/Gu; RH-II\/GU; RH-II\/GuA; RNA helicase II\/Gu alpha\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801007837353,"sku":"59278S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-59278s","provider":"Iright","version":"1.0","type":"link"}